Our research group at the AMOLF institute focuses on three themes:
Folding pathways are traditionally studied for proteins in isolation, even though chaperones are critical to achieving native folds. Consequently, the mechanisms by which chaperones act remains poorly understood. We address this question with a single-molecule approach, using optical tweezers, protein constructs, and computer modelling (Science 2007, Nature 2013).
The stochastic nature of gene expression is increasingly understood, but how it impacts growth and fitness remains unclear. We investigate this issue using genetic engineering, microfabricated flow-cells, and single-cell time-lapse fluorescence microscopy (EMBO rep. 2009, Nature 2014).
Evolutionary processes are typically studied in constant environments, and a descriptive manner. As a result, the evolutionary dynamics in variable environments has been barely addressed, even though this is considered central to the evolution of complex biological functions. Using synthetic biology and mathematical modelling, we aim to bring a more predictive approach to these fascinating issues (Nature 2007, Cell 2011).